Gas-Liquid Chromatography of Thermal Polymerization Products of Methyl Linoleate

Preparation of fatty acid methyl esters for gas-liquid chromatography.

A convenient method using commercial aqueous concentrated HCl (conc. HCl; 35%, w/w) as an acid catalyst was developed for preparation of fatty acid methyl esters (FAMEs) from sterol esters, triacylglycerols, phospholipids, and FFAs for gas-liquid chromatography (GC). An 8% (w/v) solution of HCl in methanol/water (85:15, v/v) was prepared by diluting 9.7 ml of conc. HCl with 41.5 ml of methanol....

Determination of Cholesterol in Pasta Products Using Gas-Liquid Chromatography

A simple gas-liquid chromatographic method has been developed for the measurement of cholesterol and some plant sterols, such as campesterol, ~-sitosterol and avenasterol, in pasta products. Samples were saponified, without prior extraction of lipids, and the concentrated unsaponifiables analysed directly by gas liquid chromatography. The results were evaluated statistically, and they showed th...

Gas and Liquid Chromatography

Mutagenicity tests of lipid oxidation products in Salmonella typhimurium: monohydroperoxides and secondary oxidation products of methyl linoleate and methyl linolenate.

Nine hydroperoxy and hydroperoxy-epidioxy oxidation products derived from either autoxidation (AO) or photosensitized oxidation (PO) of methyl linoleate (MLo) or methyl linolenate (MLn) were tested for mutagenic activity by the Salmonella typhimurium his+ reversion assay using strains TA100, TA98, TA102, TA97 and TA1537. All nine oxidation products, monohydroperoxides from AO-MLn (I) or from PO...

Wine: Gas and Liquid Chromatography

From the chemical point of view, wines are aqueous alcoholic solutions containing more than 1000 components that can be present at high concentrations (g L ), but also at trace levels (ng L ). Some of these components determine the organoleptic properties of wines, while others are signi"cant for classifying their origin and/or for checking whether some adulteration has taken place. Concentrati...